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| Registros recuperados: 2.860 | |
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| Cai,Jin; Gao,Yichen; Wang,Mengliang; Zhang,Jing; Zhang,Yongbin; Wang,Qi; Liu,Jiquan. |
| Abstract Clavibacter michiganensis subsp. michiganensis (Cmm), which is a Gram positive bacterium, causes the bacterial canker of tomato disease. The purpose of the study was to evaluate the antibacterial activity of Polygonum orientale extracts against Cmm. In this study, firstly, effects of three extracting parameters (extractive time, extractive temperature, and solid to liquid ratio) of orthogonal experiment design L27 (313) were conducted. Secondly, survival rate was determined and inhibition zone of Cmm rescued post-stress was monitored. Finally, extracellular OD260nm value, extracellular protein content, conformational structure of membrane protein, extracellular alkaline phosphatase (AKP) activity, and ATPase activity were measured to investigate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant bioactive compounds; Antibacterial agent; Plant pathogenic bacterium; Orthogonal experiment design; Biological control. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100217 |
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| Machado,Alexandre Paulo; Vivi,Viviane Karolina; Tavares,José Roberto; Gueiros Filho,Frederico José; Fischman,Olga. |
| In this work, different reactions in vitro between an environmental bacterial isolate and fungal species were related. The Gram-positive bacteria had terminal and subterminal endospores, presented metabolic characteristics of mesophilic and acidophilic growth, halotolerance, positive to nitrate reduction and enzyme production, as caseinase and catalase. The analysis of partial sequences containing 400 to 700 bases of the 16S ribosomal RNA gene showed identity with the genus Bacillus. However, its identity as B. subtilis was confirmed after analyses of the rpoB, gyrA, and 16S rRNA near-full-length sequences. Strong inhibitory activity of environmental microorganisms, such as Penicillium sp, Aspergillus flavus, A. niger, and phytopathogens, such as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilis; Phytopathogenic fungi; Environmental fungi; Microbial interaction. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500001 |
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| Das,Gitishree; Patra,Jayanta Kumar; Choi,Jaehyuk; Baek,Kwang-Hyun. |
| ABSTRACT Equisetum arvense, a fern species possesses a number of pharmaceutical prospective. In the present study, a total of 103 endophytic bacteria isolated from E. arvense and were evaluated for their anticandidal property against five Candida species, two C. albicans, C. glabrata, C. saitoana and C. geochares. Out of them fifty one were identified as per the morphological and molecular characterisation using 16S rRNA gene sequencing and among them, ten promising endophytic bacteria were mentioned in the present study. Among ten endophytic bacteria, Psychrobacillus insolitus and Curtobacterium oceanosedimentum exerted highest anticandidal effect against C. albicans KACC 30062 and C. glabrata KBNO6P00368, with diameter of inhibition zones of 21.30±0.41... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anticandidal effect; Endophytic bacteria; Equisetum arvense; Candida albicans; Candida glabrata. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100455 |
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| Li,Xiaofan; Song,Qingxiao; Hu1,Wanyu; Wan,Bo; Huang,Qinghua; Li,Qing. |
| ABSTRACT To establish a transfusion-associated graft-versus-host disease (TA-GVHD) mouse model with busulfan and fludarabine for effective treatment evaluation. BALB/c (H-2d) mice were injected with busulfan (15 mg/kg) and fludarabine (30 mg/kg) twice a day for 4 days. The mice were transfused with 106 T cell-depleted bone marrow (TCD-BM )and cells in different groups 3 days after chemotherapy: syngeneic BALB/c, MHC minor mismatch DBA/2 (H-2d), or MHC major mismatch C57BL/6(H2-b). Recipient BALB/c mice were injected with either blood only or blood+splenocyte. TA-GVHD was monitored in terms of body weight loss, clinical scores, and survival. Dexamethasone (50 mg/kg), cyclophosphamide (50 mg/kg), cyclosporine A (30 mg/kg), and anti-CD3 (1 mg/kg) were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transfusion-associated graft-versus-host disease; Fludarabine; Busulfan; Animal model; Major histocompatibility complex. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100305 |
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| Rodrigues,José Ariévilo Gurgel; Vanderlei,Edfranck de Sousa Oliveira; Bessa,Érika Façanha; Magalhães,Francisco de Araújo; Paula,Regina Célia Monteiro de; Lima,Vilma; Benevides,Norma Maria Barros. |
| The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marine algae; Sulfated macromolecules; Chemical analysis; APTT test. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007 |
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| Abbas,Rao Zahid; Iqbal,Zafar; Khan,Muhammad Nisar; Zafar,Muhammad Arif; Zia,Muhammad Anjum. |
| Comparative efficacy of turmeric (Curcuma longa L.) crude powder and salinomycin sodium on the occurrence of coccidiosis and growth performance of broiler was evaluated. A total of 90, day-old chicks were randomly divided into six groups. From first day onward, ration was supplemented with 1, 2 and 3 % turmeric powder in groups 2, 3 and 4, respectively, group 1 received salinomycin sodium @ 12 g 50 kg-1 feed while groups 5 and 6 were kept as infected un-medicated and uninfected un-medicated controls. First five groups were infected with Eimeria tenella sporulated oocysts @ 1,00,000/chick at the age of 20 days. Body weight gain, feed consumption, feed conversion ratio were investigated throughout the experimental period, and bloody diarrhea and oocysts... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Curcuma longa; Coccidiostat; Eimeria tenella. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100008 |
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| Zhang,Zuofa; Lv,Guoying; Pan,Huijuan; Santos,Leandro Freire dos; Fan,Menghai; Soccol,Carlos Ricardo; Thomaz-Soccol,Vanete; Lacerda,Luiz Gustavo; Carvalho Filho,Marco Aurélio da Silva; Fan,Leifa. |
| The study aimed to investigate the antihyperglycemic and antilipidperoxidative effects of ethanol extract of the dry matter from culture broth of Coriolus versicolor (ECBC) in streptozotocin-induced diabetes mice. Blood glucose level, insulin level, total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol(LDL-C), high density lipoprotein -cholesterol (HDL-C) in serum and reduced glutathione level (GSH), lipid peroxidation, glycogen, antioxidant enzymes in liver were evaluated. Moreover, histopathological observation was conducted. Streptozotocin treatment (150 mg/kg body weight) induced the decrease of GSH level, antioxidant enzymes activities, glycogen content in liver, HDL-C content and insulin level in serum, accompanied by the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coriolus versiolor; Antihyperglycemic; Antilipidperoxidative; Diabetes. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400008 |
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| Qadir,Nadir Mustafa; Ali,Khabat Anwar; Qader,Suhailah Wasman. |
| This study have been designed to study the effect of extracted pure oleuropein from Oleaeuropaealeaf against alloxan induced type 1 diabetic rats.Diabetic male ratwas induced by injectingsingle subcutaneous injection of 100 mg/kg b.wof alloxan.Respectively, pure oleuropein compound (5, 10, 15 and 20 mg/kg.b.w)was orally administered once per a day during a period of 40 days ofexperiment.Then, the serum blood was collected for the determination of glucose level,haematological analysis, enzymatic and non-enzymatic antioxidant. Further, pancreatic tissue was evaluated for histological examination. Oleuropein showed a significant rolein attenuating the blood glucose levels and elevation of in-vivo antioxidantafter treating diabetic rats with 5 and 10 mg/kg.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oleuropein; Type 1 Diabetic; Alloxan; Antioxidant; Blood glucose and HBA1C. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100322 |
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| Pal,Savita; Mishra,Arvind; Rawat,Arun Kumar; Gautam,Sudeep; Maurya,Rakesh; Srivastava,Arvind K.. |
| The present study was undertaken to investigate the antidiabetic potential of tap roots of Potentilla fulgens in streptozotocin induced diabetic rat models. The crude powder, ethanolic, ethanolic: aqueous and aqueous extracts of tap roots were administered to normoglycemic- and streptozotocin (STZ)-induced diabetic rats in a single dose study. The ethanolic extract showed significant improvement in oral glucose tolerance and antihyperglycemic effect on sucrose loaded normal rats and STZ-induced diabetic rats. Of the isolated aqueous, n-butanol, chloroform and n-hexane soluble fractions of the active ethanolic extract of the roots, the aqueous fraction (100 mg/kg body weight) showed significant blood glucose lowering effect on STZ-induced diabetic rats. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antidiabetic activity; Antihyperglycemic Activity; Antidyslipidemic activity; Potentilla fulgens roots; Alpha (α)-glucosidase enzyme. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100318 |
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| Ventura,Maurício Ursi; Ito,Márcio. |
| Studies were carried out to determine the antifeedant activity of extracts of leaves, stems, flowers and fruits of Melia azedarach (L.), the dosage activity responses to the most active extract and the effects of sunlight in the activity persistence to Diabrotica speciosa (Genn.) beetles. Extracts efficiency was determined by evaluating leaf consumption. Insect feeding was deterred by stems, fruits and flowers extracts. Flowers and fruits extracts were the most efficient. Stems extract was in an intermediate position between the two most efficient and the least one (leaf extracts). The most active extract (flowers) was sprayed at concentrations of 0, 1, 2, 3, 4, 5, 6 and 7 g/100 ml. Feeding of D. speciosa on common bean leaves extracts decreased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Insecta; Leaf beetle; Phaeolus vulgaris (L.); Deterrence; Botanical insecticide. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200011 |
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| Faria,Terezinha de Jesus; Ferreira,Rafael Sottero; Yassumoto,Lidiane; Souza,José Roberto Pinto de; Ishikawa,Noemia Kazue; Barbosa,Aneli de Melo. |
| An investigation of antifungal activity of the essential oil obtained by steam-distillation (1.1% w/w) of the aerial parts of Ocimum gratissimum and of an ethanolic extract from the steam-distillation residue was carried out using the agar diffusion method. The results revealed that the essential oil inhibited the growth of all fungi tested, including the phytopathogens, Botryosphaeria rhodina, Rhizoctonia sp. and two strains of Alternaria sp., while the extract from the residue was inactive. The essential oil was subjected to TLC bioautography used to detect fungitoxic constituents. The compound that showed antifungal activity was isolated and identified as eugenol. GC/MS analysis showed that eugenol was the main constituent of the essential oil studied.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ocimum gratissimum; Antifungal activity; Eugenol; Phytopathogenic fungi. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700002 |
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| Abid,Muhammad; Chohan,Sobia; Mehmood,Mirza Abid; Naz,Safina; Naqvi,Syed Atif Hasan. |
| ABSTRACT Bitter gourd is of great importance due to its usage against the treatment of numerous ailments in human beings. A comprehensive survey at four localities of Southern Punjab, Pakistan was carried out to determine the severity of Myrothecium leaf spot. Maximum disease severity was at C1 (Chak 11/NP) and least at C2 (Kot Mehtab). Among isolated species Myrothecium roridum was found more prevalent and pathogenic as compared to M. verrucaria. Antifungal activity using solvent extracts of five medicinal plants (Mangifera indica, Melia azedarach, Nicotiana tabacum, Moringa oleifera and Eucalyptus globosum) were evaluated against isolated species by agar well diffusion method at various concentrations (0.01, 0.10, 1.0 and 10.0 µg / mL). N. tabacum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Momordica charantia L.; Secondary compounds; Solvent plant extracts. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100421 |
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| Camilo,Simone B.; Ono,Carlos José; Ueno,Yoshio; Hirooka,Elisa Y.. |
| Studies were carried out to isolate microorganisms from corn and silage screened for their ability to inhibit F. moniliforme growth (strain 113F) in association with fumonisin detoxification. Among 150 isolates four Gram-positive bacilli and one yeast with inhibitory activity were selected. The inhibition zone ranged from 50 to 72.5 mm using cultures, and from 25 to 52.5mm for crude alcoholic extract. The isolates S9, S10, S69 (sporulated bacilli) and SE3071 (yeast) degraded 43, 48, 83 and 57% of the initial FB1 concentration, respectively. The pH increased gradually in the medium during incubation for biodegradation process. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fusarium moniliforme; Fumonisin; Biodegradation. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000200004 |
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| Monteiro,Jessica Raquel Borges; Ardisson,Juliana Santa; Athaydes,Brena Ramos; Gonçalves,Rita de Cássia Ribeiro; Rodrigues,Ricardo Pereira; Kuster,Ricardo Machado; Kitagawa,Rodrigo Rezende. |
| Abstract Helicobacter pylori is a bacterium that reaches half of the world population and it’s recognized as the main cause of chronic gastritis and peptic ulcer. In this study, we evaluated the anti-H. pylori, antioxidant and immunomodulatory activities of the methanolic (MeOH) extract of Eugenia uniflora leaves and chemical profile. Anti-H. pylori activity was evaluated by spectrophotometric broth microdilution technique by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), in addition to the evaluation of the effect on the urease enzyme. The antioxidant activity was evaluated by capturing O2 •-, HOCl e NO• radicals. The immunomodulatory effect was evaluated on the cytokines TNF-α, IL-6 and on nitric... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eugenia uniflora; Helicobacter pylori; Antioxidant; Immunomodulation. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100424 |
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| Registros recuperados: 2.860 | |
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